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SPR Screening of metal-chelating peptides in sunflower hydrolysates for their antioxidant properties

ABG-87393 Master internship 6 months 560 euros/month
2019-09-12
Centre National de la Recherche Scientifique
Nancy Grand Est France
  • Biotechnology
  • Biochemistry
SPR screening, metal-chelating peptides, antioxidants, sun-flower hydrolysates.
2019-10-31

Employer organisation

The trainee will be localised at LRGP- ENSIC and will make part of his/her experiments at Biopole of Nancy.

Description

SPR Screening of metal-chelating peptides in sunflower hydrolysates for their antioxidant properties

 

Antioxidants are widely used in health, cosmetic, and nutrition applications. As some synthetic antioxidants are suspected to be hazardous for human health, there is a need for new biomolecules presenting such bioactivities. The antioxidant capacity of molecules is closely linked to the scavenging of free radicals and the inhibition of lipid peroxidation. Peptides are interesting tools as some of them, depending on their composition, were shown to be able to chelate iron(II), inhibiting iron oxidation through Fenton reaction. This chelation power of peptides is an indirect physiological mechanism that in return inhibits radical chain reactions and delays oxidation phenomena.

To date, the discovery of new bioactive molecules is generally based on systematic empirical approaches, i.e., several cycles of separation/fractionation followed by biological tests until identification of the peptide sequence by mass spectrometry (MS/MS). Such approaches suffer many drawbacks like fastidious, expensive, uncertainty to obtain bioactive peptides or low concentration of peptide of interest produced at small scale. Therefore new screening methods with high sensitivity should be considered before launching time-consuming separation of a complex mixture of peptides.

This master 2 research aims at discovering and testing new potential chelating antioxidant peptides from sunflower hydrolysates.

Task 1. Producing sunflower hydrolysates from sunflower proteins (LRGP). In the first step, various sunflower hydrolysates will be produced from sunflower protein isolate using on various parameters (Enzyme, E/S ratio, individual or sequential treatment). Then, the produced hydrolysate will be investigated for their antioxidant properties using metal-chelation tests according to the methodologies developed in Canabady-Rochelle et al (2015).

Task 2. Screening of Metal-Chelating Peptides in sunflower hydrolysates using the SPR methodology previously developed on Ni(II) (Canabady-Rochelle et al, 2018). This SPR methodology, initiated on commercial hydrolysates and on synthetic peptides (Canabady-Rochelle et al, 2016 and 2017, 2018), is currently under application for soy-protein laboratory hydrolysates. This screening will determine by SPR the best biocatalysis conditions for producing metal-chelating peptides in sunflower hydrolysate

Task 3. Finally, the best hydrolysates in terms of metal chelation properties identified in SPR will be will be characterized for their metal-chelating sequences in Mass spectrometry.

 

Experiments will be mainly carried out between LRGP and IMOPA.

 

References

Canabady-Rochelle, et al. 2016, 17th International Conference on Oxidative Stress Reduction, Redox Homeostasis and Antioxidants June 1315, Institut Pasteur, Paris.

Laetitia L.S. Canabady-Rochelle, Katalin Selmeczi, Sabrina Collin, Andreea Pacs, Laurence Muhr and Sandrine Boschi-Muller. (2018) SPR Screening of Metal chelating Peptides for their Antioxidant Properties. Food Chemistry. 239, 478-485.

Canabady-Rochelle, et al. (2015). Determination of reducing power and metal chelating ability of antioxidant peptides: revisited methods. Food Chem. 183, 129-135.

 

Key words: SPR screening, metal-chelating peptides, antioxidants, sun-flower hydrolysates.

 

 

To apply, please send the following documents. The student must be able to communicate in English with her/his supervisor.
- CV and Motivation Letter
- Notes of your last year before M2
 

 

 

Profile

The candidate should be ideally a biochemist with skills in enzymology.

He/She must be able to communicate in english.

Starting date

2020-01-06
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