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Post-doctoral position on mobile DNA, genome instability and organization

ABG-107920 Emploi Confirmé
11/01/2023 CDD Salaire à négocier
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CNRS-INSERM- Université Paris Cité
PARIS - Ile-de-France - France
Biologie
  • Biochimie
  • Informatique
Transposable elements, Chromosomes dynamics , microscopy, genome wide analyses , budding yeast
Recherche et Développement

Employeur

An application is open for a Postdoctoral Research position in the laboratory of Dr. Emmanuelle Fabre and Pascale Lesage, located in the heart of Paris to undertake a basic  research project to characterize the mechanisms involved in the establishment of genome instability triggered by transposable elements in the context of 3D genome organization .

The Institut national de la santé et de la recherche médicale (National institute for Health and Medical Research, INSERM) is a public organization under the responsibility of the French Ministry of Higher Education and Research. INSERM laboratories (or research units) are located throughout France, and employ a large body of tenured researchers, engineers, and support staff. Our laboratory is part of a research unit which is partnered with Paris Cité University, a major French university, and is settled in the St Louis Hospital a historic hospital embedded in a highly dense research and clinical campus providing interactions with many experts in the field. We are located in the north east of Paris, a dynamic  district, surrounded by all shops, historical monuments and public transportation. Our team is a creative and stimulating place to work. We will recognize talent and support career advancement. 

Our team "Genome biology: from mobile DNA to chromosome dynamics" has a long experience in the functional organization of chromosomes in nuclear space and in the biology of transposable elements. Our interdisciplinary team has expertise in both genome-wide analyses of eukaryotic yeast genome  and single-cell dynamic microscopy, which allows for a powerful cross-talk to address the question of genomic instability associated with mobile elements in the context of the 3D organization of chromosomes.

A 24-month renewable contract (on new grant  application) will be established according to the French Labour Code and includes a full social security scheme (sickness, maternity benefits, pension contribution, etc..). Salary depends on experience. The INSERM is committed to implement fair and transparent recruitment and appraisal procedures for researchers. Position is available from January 2023 (open until filled).

Poste et missions

You have a PhD in the biological or biochemical sciences with demonstrated experience in molecular and cellular biology techniques. You have proven experience in carrying out projects from conception to completion as well as strong organizational, teamwork and communication skills. Your CV and publications are competitive for international postdoctoral fellowships. Candidates with experience in yeast biology, genome-wide experimental skills, and knowledge of bioinformatics analyses of high-throughput imaging data will be appreciated.

 

To understand the impact of 3D chromosome organization on (TEs)-mediated genome rearrangements, the postdoctoral project will include the following questions: What induces TE contacts in the nuclear space? Which consequences have these contacts on TE-mediated genome rearrangements? How stress conditions do impact TE contacts and TE-mediated rearrangements?

 

You will combine state-of-the-art sequencing and live microscopy approaches with molecular biology techniques, and use budding yeast as an experimental system.

Mobilité géographique :

Européenne

Télétravail :

Occasionnel

Profil

Skills

  • University degree (Ph.D.), with a strong specialization in molecular and cellular biology techniques.
  • Demonstrated experience in carrying out projects from conception to completion, first author paper of an original publication is mandatory.
  • Strong organizational, teamwork, synthesis and communication skills.
  •  Ability to work in a team, supervisory experience may be a plus.
  • Ability to communicate in English.

Objectifs

Genomic research has reached a milestone with two decades of high-throughput sequencing data, leading to the characterization of the primary sequence of genomes and the first descriptions of their 3D organization in cellular space. It is fascinating that genomes are full of dispersed, repeated and potentially mobile sequences, that the expression of a genome depends largely on its three-dimensional organization in the nucleus and that subtle changes in genome linearity are the source of rapid and reversible adaptation of cells to their environment. Yet how transposable elements and 3D genome conformation together drive cell fate decisions remains an enigma.

 

Our research aims at dissecting the molecular mechanisms involved in the recombination of transposable elements (TEs) during environmental stresses leading to adaptation, with a specific focus on the role played by genome organization. We combine state-of-the-art sequencing and live microscopy approaches with molecular biology techniques, using budding yeast as an experimental system.

 

The postdoctoral fellow will focus on the impact of 3D organization on chromosomal translocations due to mismatched DNA double-strand breaks induced at Ty1 transposable elements. The postdoc will define Ty1 translocation rates, map Ty1 genomic contacts under normal and stressful conditions, and reveal the impact of 3D chromosome organization in this process.

 

Recent publications from the host lab linked to the project:

Bridier-Nahmias, A. et al. Science. 348, 585–588 (2015).

Herbert, S. et al. EMBO J. 36,doi: 10.15252/embj.201695842 (2017).

Asif-Laidin, A. et al. EMBO J. 39, 1–17 doi:10.15252/embj.2019104337 (2020).

Garcia Fernandez, F. et al. J. Cell Sci. doi: 10.1242/jcs.258500  (2021).

Garcia Fernandez, F. et aleLife, doi:10.7554/eLife.78015 (2022).

Nguyen P Q, et al. bioRxiv 2022.03.07.483246 (2022), under revision at Nature communications

Some recent reviews

Sultana, T. et al. Nat. Rev. Genet. 18, 292–308 (2017).

Zimmer, C. & Fabre, E. Curr. Genet. 18, 1200 (2019).

Bonnet, A. & Lesage, P. Curr. Genet. 67, 347–357 (2021).

García Fernández & Fabre, E. Genes . 3(2):215 (2022).

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