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“CHEMICAL IDENTIFICATION AND BIOSTIMULANT PROPERTIES OF METABOLITES PRODUCED BY MICROBIAL STRAINS ISOLATED FROM BROWN SEAWEEDS”

ABG-99386 Stage master 2 / Ingénieur 6 mois Selon grille de gratification Master 2
21/07/2021
National Museum of Natural History
Paris Ile-de-France France
  • Chimie
  • Agronomie, agroalimentaire
03/01/2022

Établissement recruteur

Workplace : Natural History Museum, Paris (75 %) and Saint Malo (25 %) (Agro Innovation International, CMI Roullier - Saint-Malo).

 

 

Description

Context: this Master 2 internship will be carried out in the context of the SEABIOZ ANR project involving both academic partners (Roscoff Biological Station, IRISA - Rennes, Natural History Museum - Paris) and an industrial partner (Agro Innovation International, CMI Roullier - Saint-Malo). This 4-year project, started in 2021, aims at providing new insights in the Ascophyllum nodosum holobiont by evaluating the impact of season, origin, and microbial communities on biological properties of seaweed extracts. A. nodosum is a common brown alga found along the coasts of the North Atlantic Ocean. Very little is known so far about the importance of these symbionts for the seaweed, except that they are believed to protect A. nodosum germlings from desiccation and improve their robustness to different stresses. A. nodosum also constitutes a prominent raw material used for many years to produce plant biostimulants and solutions for the agriculture. Different bioactive molecules such as original polysaccharides (laminarin, fucoidans, alginates), mannitol, betaines, polyamines, and polyphenolic compounds, could be involved in these properties but the chemical nature and the biosynthetical origins of the active molecules remain unknown.

Internship goals: The main objective of the internship will be to investigate the chemistry and the biological activities of metabolites isolated from microbial strains associated with A. nodosum. These isolated microbial strains will be cultivated in specific media appropriate for fungi and bacteria development according to standard protocols developed by MNHN. The microbial crude extracts will be produced following MNHN protocols to maximize the recovery of a large number of metabolites and following the AII Roullier requirements which has a restrictive list of solvent and cost constraints linked to the process upscaling. The analytical annotation of those fungal and bacterial metabolites will be performed by several mass spectrometry tools developed by MNHN and AII Roullier including metabolomic and molecular networking This approach will be combined with research in natural products and in-house databases.
In vitro bioassays will be carried out at the CMI Roullier to determine the most active crude extracts for biostimulant properties. Compounds will be thus specifically isolated from active crude extracts obtained from large-scale cultivation of microorganisms. Pure compounds will be obtained by different purification techniques (precipitation, ultrafiltration, preparative chromotagraphy, SPE, …) and then fully characterized by Nuclear Magnetic Resonance (NMR) and MS spectroscopies.

 

 

 

Profil

Student profile: The recruited Master 2 student will have strong experience working at the microbiology / chemistry of natural substances interface and will have a strong interest in fundamental research and in applied research.
• Chemistry: experience in chemistry of natural substances: Isolation and characterization of molecules by MS, NMR Microbiology: knowledge or interest in microbiology will be an asset.
• Biology: knowledge or interest in plant physiology/biology will be an asset
• Communication skills: english nglish nglish nglish skills, writing of regular reports
Additional Information

Prise de fonction

01/07/2022
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