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Role of Hedgehog pathway in myogenic differentiation

ABG-133851 Master internship 6 months 4,35 euros/h
2025-10-15
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Université Paris Est - Créteil
Ile-de-France France
  • Biology
2025-10-31

Employer organisation

Biology of the neuromuscular system, IMRB INSERM U955, Creteil, France

https://imrb.inserm.fr/en/home/

Description

Research topic:

The project aims to decipher the role of primary cilium and primary cilium-mediated Hedgehog signaling during myogenic differentiation. In collaboration with Henri Mondor University Hospital, we use primary human myoblasts obtained from patients undergoing diagnostic muscle biopsies (in compliance with ethical regulations and informed consent). These cells are induced to differentiate and fuse in vitro. Using immunofluorescence and confocal microscopy, RT-qPCR, Western blot, and RNA-seq, we investigate how primary cilium contributes to skeletal muscle lineage commitment. Our preliminary results indicate activation of the Hedgehog pathway at the onset of myogenic differentiation. We now aim to confirm this by modulating the pathway in vitro and analyzing its effects on muscle cell fate.

Profile

We are seeking a motivated candidate with:

Enrollment in a relevant degree program (biology, biomedical sciences, biotechnology, etc.)

Proficiency in English, scientific rigor, and strong organizational skills

Familiarity with data analysis tools and basic statistics (Excel, GraphPad)

Prior experience in at least one of the following techniques is a plus: cell culture, RT-qPCR, microscopy, Western blot 

Starting date

2026-01-01
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